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TEM
Hi
I have to disagree with Paul:
>I don't think that you can make stereo with a transmission electron
>microscope. What you need is a scanning electron microscope (SEM).
>Then you rotate the subject about 6-degrees between exposures. Cut
>out the images of the subject on the print paper and mount them on a
>black background, or do same thing with PhotoShop. Works
>beautifully.
>
>I don't think that a TEM has enough depth to work with.
You can have a lot of depth with freeze-fracture replicas in the TEM. If
you ever have the opportunity to see some replicas, you will be very
impressed.
Yes you can some depth in thin sections. Normally we would tilt our
specimens up to about +10/-10 degrees on the stage. It depends on the
specimen. Experimenting with your specimens is the best way.
Yes SEM images are great. Would love to have one at home. Unfortunately my
family would end up fighting over it.
Best wishes
Mark Gould
xxxx x x x Southernmost E.M Unit in the World!
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xxxxxx x x Mark Gould
xxxxxxx x x P.O Box 913
xxxxxxx x x Otago Medical School
xxxxxx x x C/- Anatomy and Structural Biology
xxxxx x x x Dunedin
xxxx x x x Ph. (03) 4797301
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(Yellow eyed penguin)
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